Dr. Hank W. Bass
HW Bass Faculty Page
Bass Lab Home Page
Ph.D., North Carolina State University, 1992.
Graduate Faculty Status
We are investigating the structure and function of chromosomes during meiosis, when chromosomes are recombined, shuffled, and reduced to the haploid state (during production of sperm or eggs). High-resolution deconvolution microscopy is used to collect multi-wavelength three-dimensional optical reconstructions of whole nuclei in meiotic cells of higher plants. Specific DNA sequences are localized within the nucleus by fluorescence in situ hybridization (FISH), and sophisticated software is used to analyze the resulting spatial and quantitative image data. We are currently working on the analysis of meiotic telomere and nuclear envelope functions and chromatin structural genomics in maize.Selected Publications:
(2016) Rodgers-Melnick E, Vera DL, Bass HW, and Buckler ES. "Open Chromatin Reveals the Functional Maize Genome." Proc Nat Acad Sci, USA 113(22):E3177-E3184 (doi: 10.1073/pnas.1525244113).
(2015) Kopylov M, Bass HW, and Stroupe ME. "The maize (Zea mays L.) nucleoside diphosphate kinase1 (ZmNDPK1) gene encodes a human NM23-H2 homologue that binds and stabilizes G-quadruplex DNA" Biochemistry 54(9):1743-1757 (DOI: 10.1021/bi501284g)
(2014) Vera DL, Madzima TF, Labonne JD, Alam MP, Hoffman GG, Girimurugan SB, Zhang J, McGinnis KM, Dennis JH, and Bass HW. "Differential nuclease sensitivity profiling of chromatin reveals biochemical footprints coupled to gene expression and functional DNA elements in maize." Plant Cell 26(10):3883-3893.
(2014) Andorf CM, Kopylov MS, Dobbs D, Koch KE, Stroupe ME, Lawrence CJ, and Bass HW. "G-quadruplex motifs in maize (Zea mays L.) occupy specific sites in thousands of genes coupled to energy status, hypoxia, low sugar, and nutrient deprivation" J Genetics Genomics (JGG) 41(12):627-647. (DOI: 10.1016/j.jgg.2014.10.004).
(2014) Murphy SP, Gumber HK, Mao YY, and Bass HW. "A dynamic meiotic SUN belt includes the zygotene-stage telomere bouquet and is disrupted in chromos ome segregation mutants of maize (Zea mays L.)." Front Plant Sci. 5:314. (doi: 10.3389/fpls.2014.00314).
(2014) Bass HW, Wear EE, Lee T-J, Hoffman GG, Gumber HK, Allen GC, Thompson WF, Hanley-Bowdoin L. "A maize root tip system to study DNA replication programmes in somatic and endocycling nuclei durin g plant development" J Exp Botany 65:2747-2756.
(2013) Fincher JA, Vera DL, Hughes DD, McGinnis KM, Dennis JH, and Bass HW. "Genome-wide prediction of nucleosome occupancy in maize (Zea mays L.) highlights chromatin structural features at multiple scales." Plant Physiol 162(2):1127-1141.
(2013) Graumann K, Bass HW, and Parry G. "SUNrises on the International Plant Nucleus Consortium: SEB Salzburg 2012" Nucleus 4(1):3-7 dx.doi.org/10.4161/nucl.23385.
(2012) Howe ES, Clemente TE, and Bass HW. "Maize histone H2B-mCherry, a new fluorescent chromatin marker for somatic and meiotic chromosome re search." DNA and Cell Biology. 31(6):925-938. (DOI: 10.1089/dna.2011.1514).
(2012) Figueroa DM and Bass HW. "Development of pachytene FISH maps for six maize chromosomes and their integration with other maize maps for insights into genome structure variation." Chromosome Research 20(4):363-380. (DOI: 10.1007/s10577-012-9281-4)
(2012) Murphy SP and Bass HW. "The maize (Zea mays L.) desynaptic (dy) mutation defines a pathway for meiotic chromosome segregation linking nuclear morphology, telomere distribution, and synapsis." J Cell Science 125(15):3681-3690. (doi: 10.1242/jcs.108290)
(2011) Brown AN, Lauter N, Vera DL, McLaughlin-Large KA, Steele TM, Fredette NC, and Bass HW. "QTL mapping and candidate gene expression analysis of telomere length control factors in maize." (Zea mays L.). G3: Genes, Genomes, Genetics 2011 Nov;1(6):437-450. (doi:10.1534/g3.111.000703)
(2011)Huang H, Besiki S, Jorgensen C, and Bass HW. "Prioritization of data quality dimensions and skills requirements in genome annotation work." J Am Soc Info Sci and Technol 63:195-207. ( doi: 10.1002/asi.21652).
(2011) Figueroa DM, Davis JD, Strobel CE, Conejo MS, Beckham KD, Ring BC, and Bass HW. "Sorghum (Sorghum propinquum) Bacterial Artificial Chromosomes as Cytogenetic FISH Probes for Maize (Zea mays L.)." J Biomed Biotechnol vol. 2011, Article ID 386862, 16 pages (doi:10.1155/2011/386862).
(2010) Murphy SP, Simmons C, and Bass HW. "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants." BMC Plant Biology 10:269.
(2010) Figueroa DM and Bass HW. "A Historical and Modern Perspective on Plant Cytogenetics." Briefings in Functional Genomics and Proteomics 9(2):95-102.