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Ammonium Acetate Precipitation

This protocol is useful for cleaning up DNA obtained from mini-prep kits. The procedure is as follows:

1. Bring volume up to 100 microliters with water.



2. Add 10 microliters of 5 M Ammonium Acetate and 280 microliters of 95% Ethanol.



3. Vortex and place at -20 degrees Celsius for 20 minutes.



4. Spin 5 minutes at room temperature in a micro-centrifuge and remove supernatant.
   
   
5. Add 250 microliters of 70% Ethanol and mix thoroughly.



6. Spin 5 minutes at room temperature in a micro-centrifuge and remove supernatant.
   
   
7. Dry in speedvac.



8. Resuspend in water.



9. Take O.D. to determine concentration.