BIOLOGICAL SCIENCEFACULTY MEMBER Dr. David M. Gilbert
Laboratory Home Page  Replication Domain Database J. Herbert Taylor Distinguished Professor of Molecular Biology Ph.D., Stanford University, 1989 Research and Professional Interests: Our overall goal is to understand how mammalian chromosomes are organized within the nucleus to carry out their various functions. DNA replication provides an excellent forum with which to study chromosome structure and function. Structural and functional units of chromosomes replicate coordinately, often through the synchronous firing of clusters of replication origins that encompass domains of approximately 1Mb. Each of these replication domains is programmed to replicate at a specific time during S-phase. In general, transcriptionally active (euchromatin) domains replicate early in S-phase and transcriptionally silent (heterochromatin) domains replicate late. Programmed changes in replication timing accompany key stages of metazoan development and are often coupled to changes in gene expression. These findings suggest that structural, functional, and replication domains share topographical boundaries and represent basic units of chromosome organization. We would like to understand what regulates where and when replication initiates and, ultimately, how developmental cues communicate with the cell-cycle machinery to elicit changes in the program for replication. Selected Publications (last 3 years): Gilbert, D.M. (2007) Replication Origin Plasticity, Taylor-Made: inhibition vs. recruitment of new origins under conditions of replication stress. CHROMOSOMA, 116: 341-347. Sasaki, T. and Gilbert, D.M. (2007) The Many Faces of the Origin Recognition complex. CURR. OPIN. CELL BIOL. 19:337-343. Gilbert, D.M. and Zink, D. (2007) Intranuclear Changes in Cancer Cells. GENOME BIOLOGY 8:312. Lu J, Gilbert DM. (2007) Proliferation-Dependent and Cell-Cycle Regulated Transcription of Mouse Peri-centric Heterochromatin. J. CELL BIOL. 179: 411-421. Yokochi, T. and Gilbert, D.M. (2007) Replication Labeling with Halogenated Thymidine Analogs. CURRENT PROTOCOLS IN CELL BIOLOGY 22.20.1.22.10.14 Lu J. and Gilbert, D.M. (2008) Cell Cycle Regulated Transcription of Heterochromatin in Mammals vs. Fission Yeast: functional conservation or coincidence? CELL CYCLE 7: 1907-1910 Hiratani, I, Ryba, T, Itoh, M. Yokochi, T., Schwaiger, M., Chang, C-W, Lyou, Y. Townes, T.M., Schubeler, D. and Gilbert, D.M. (2008) Global Re-organization of Replication Domains During Embryonic Stem Cell Differentiation PLoS BIOLOGY 6: e25. Weddington, N., Stuy, A., Hiratani, I. Ryba, T., Yokochi, T. and Gilbert, D.M. (2008) ReplicationDomain: a visualization tool and comparative database for genome-wide replication timing data. BMC Bioinformatics, 9:530 Gilbert, D.M. (2009) Establishment of a Spatial and Temporal Program for Mammalian Chromosome Replication. PROTEIN, NUCLEIC ACID AND ENZYME 54: 320-326. Hiratani, I. and Gilbert, D.M. (2009) Replication Timing as a Novel Epigenetic Mark. EPIGENETICS, 4: 93-97. Hiratani, I., Takebayashi, S., Lu, J. and Gilbert, D.M. (2009) Replication timing and transcriptional control: beyond cause and effect. Part II. CURR. OPIN. GEN. DEV. 19: 1-8 Postdoctoral Associates:Hiratani, Ichiro Graduate Students: Battaglia, Dana |
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Dr. David M. Gilbert - FSU Biological Science Faculty Member