BIOLOGICAL SCIENCEFACULTY MEMBER Dr. Brian P. Chadwick
Assistant Professor; Ph.D., University College London, 1997 POSITIONS AVAILABLE for graduate and undergraduate students (starting in fall of 2009). Research and Professional Interests: X chromosome inactivation (XCI) is the mammalian form of dosage compensation that serves to balance the levels of X-linked gene expression between the sexes. Early in development, one of the two X chromosomes in females is chosen to become the inactive X chromosome (Xi). Soon thereafter a cascade of events repackage the chosen X into heterochromatin, essentially shutting down gene expression along the length of the chromosome. This altered state is faithfully maintained throughout all subsequent somatic cell divisions, therefore making XCI a classical model of epigenetic gene regulation. My lab is interested in the organization and maintenance of chromatin on the Xi. My current research focuses on a large variable-number tandem repeat or macrosatellite (DXZ4) that adopts an unusual chromatin organization on the X chromosome. We are exploring how the packaging of DXZ4 influences the organization and maintenance of flanking chromatin on both the active X and Xi. Selected Publications:Chadwick B. P. 2009. Macrosatellite epigenetics: the two faces of DXZ4 and D4Z4. Chromosoma e-pub ahead of print. Chadwick, B. P. 2008. DXZ4 chromatin adopts an opposing conformation to that of the surrounding chromosome and acquires a novel inactive X specific role involving CTCF and anti-sense transcripts. Genome Research 18:1259-1269. Majumder, P., J. A. Gomez, B. P. Chadwick, and J. M. Boss. 2008. The insulator factor CTCF controls MHC class II gene expression and is required for the formation of long-distance chromatin interactions. Journal of Experimental Medicine 205:785-798. Chadwick, B. P. 2007. Variation in Xi chromatin organization and correlation of the H3K27me3 chromatin territories to transcribed sequences by microarray analysis. Chromosoma 116:147-157. Xiao, C., J. A. Sharp, M. Kawahara, A. R. Davalos, M. J. Difilippantonio, Y. Hu, W. Li, L. Cao, K. Buetow, T. Ried, B. P. Chadwick, C. X. Deng, and B. Panning. 2007. The XIST noncoding RNA functions independently of BRCA1 in X inactivation. Cell 128:977-989. Abbott, D. W., B. P. Chadwick, A. A. Thambirajah, and J. Ausio. 2005. Beyond the Xi: MacroH2A chromatin distribution and post-translational modification in an avian system. Journal of Biological Chemistry 280:16437-16445. Chadwick, B. P., and T. F. Lane. 2005. BRCA1 associates with the Xi in late S-phase, coupled with transient H2AX phosphorylation. Chromosoma 114:432-439. Chadwick, B. P., and H. F. Willard. 2004. Multiple spatially distinct types of facultative heterochromatin on the human inactive X chromosome. Proceedings of the National Academy of Sciences of the USA 101:17450-17455. Chadwick, B. P., and H. F. Willard. 2003. Chromatin of the Barr body is differentially histone methylated and enriched for HP1, histone H1 and HMG-I/Y, while many chromatin proteins are largely excluded. Human Molecular Genetics 12:2167-2178. Chadwick, B. P., and H. F. Willard. 2002. Cell cycle-dependent localization of macroH2A in chromatin of the inactive X chromosome. Journal of Cell Biology 157:1113-1123. Chadwick, B. P., and H. F. Willard. 2001. A novel chromatin protein, distantly related to histone H2A, is largely excluded from the inactive X chromosome. Journal of Cell Biology 152:375-384. |
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Dr. Brian P. Chadwick—FSU Biological Science Faculty Member
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