Patch-clamp Electrophysiology and Site-directed Mutagenesis

Lecture Outline: I. General Background on Bioelectric Signals II. Acquisition of Data III. Biological Preparations IV. Parallel Tools A. Heterologous Expression Systems B. Site-directed Mutagenesis C. Protein Biochemistry Literature Resources: You may check any of these out from my office library.... Literature is listed in order of increasing difficulty. Levitan and Kaczmarek. 1997. The Neuron. Oxford Press. New York, New York. Axon Instruments. 1993. The Axon Guide for Electrophysiology and Biophysics Laboratory Techniques. Foster City, CA. Gerrish, Dugger, and Roberts. 1999. Electricity and Electronics. Goodheart-Wilcox Company, Inc. USA. Hille, B. 2001. Ionic Channels of Excitable Membranes. Sinauer Associates. Sunderland, MA. Sakmann and Neher. 1995. Single-channel Recording. Second Edition. Plenum Press. New York, New York. Powerpoint Visuals (for screen viewing): Slide Set 1

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To Down Load the Powerpoint File (for Printing): Powerpoint Slide Set 1

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