Date:

Lecture#

Topic:

Text:

24 Aug T

1

Introduction

Basic Electricity

Hille Chapter 1;

 

Primary Literature

 

 

Course Overview; Selection of Assignments

definitions and calculations for electricity

 

27 Aug F

 

2

Electrical Circuits

Gerrish et al.

Chapters 1 & 6

 

 

definitions and calculations for circuits

 

31 Aug T

 

3

Measuring Biological and Optical Signals

Aidley

Chapters 1-3

 

 

 

 

amplifiers and voltage-clamp circuits, definitions of optical probes and equipment

 

 

3 Sept F

 

4

Ion Movement in Excitable Cells

Hille Chapter 2

 

 

Nernst potential; Goldman-Hodgkin-Katz, physical laws that dictate ion movement

 

7 Sept T

 

5

Electrical Properties of the Excitable Membrane

Hille Chapter 3

 

 

biological circuits, membrane conductance, parallel conductance model, IV relationships

 

10 Sept F

 

6

Channel Presentation #1

Discussion Leader: Lily Barret

Primary Literature;

Hille Chapter 6

 

 

 

 

Potassium Channels

 

 

14 Sept T

7

Electrical Properties of the Excitable Membrane

Hille Chapter 3

 

 

parallel conductance model, IV relationships

 

17 Sept F

 

8

Channel Presentation #2

Discussion Leader: Emily Moriarty-Lemon

Primary Literature;

Hille Chapter 7

 

 

 

 

Transmitter or Ligand-gated Channels

 

21 Sept T

 

9

 

Functional Properties of Dendrites

Primary Literature

 

 

 

 

electrotonic properties, nonisopotential cells (cylinders), rall model for neurons

 

24 Sept F

 

10

 

Channel Presentation #3

 

Discussion Leaders: Meizhu Qi

Primary Literature;

Hille Chapter 4

 

 

 

 

Sodium Ion Channels

 

 

 

28 Sept T

 

11

Nonlinear Properties of Excitable Membranes

 

Hille Chapter 2

 

 

membrane rectification, Hodgkin & Huxley gate model, constant field model

 

 

1 Oct F

 

12

Channel Presentation #4

Discussion Leaders: Destinee Gatlin

Primary Literature;

Hille Chapter 3, 9

 

 

 

 

Calcium and Non-selective Ion Channels

 

 

5 Oct T

13

Squid Giant Axon

classical voltage-clamp, H & H model, propagating & nonpropagating AP, gating currents

8 Oct F

14

Channel Presentation #5

Trp Channels and Nociceptors

Discussion Leaders: Ryan Ochoa

Primary Literature;

Hille Chapter 5

12 Oct T

 

15

 

Channel Presentation #6

Discussion Leader: Ashley Loeven

 

Primary Literature;

Hille Chapter 4

 

 

 

 

Aquaporins

 

15 Oct F

 

16

Biophysical Methods Presentation #1

Primary Literature

 

 

 

 

Patch-clamp Electrophysiology

 

Discussion Leader: Debi Fadool

 

19 Oct T

 

17

Molecular Structure and Unitary Currents of Ion Channels

Hille Chapter 13,

Sakmann/Neher Chapter 3,18, and 19

 

 

 

 

molecular structure, patch-clamp records of single-channel currents

 

 

22 Oct F

18

Stochastic Analysis of Single-Channel Function I

Sakmann/Neher Chapter

18 and 19

 

 

 

statistical analysis of single channel records, probability density functions, Markov processes, kinetic schemes, channel agonists & antagonists

 

 

26 Oct T

19

Stochastic Analysis of Single-Channel Function II

Sakmann/Neher Chapter

18 and 19

 

 

 

 

statistical analysis of single channel records, probability density functions, Markov processes, kinetic schemes, channel agonists & antagonists

 

 

29 Oct F

 

20

Biophysical Methods Presentation #2

Discussion Leader: Destinee Gatlin

Primary Literature

 

 

 

 

DREADDs

 

2 Nov T

 

21

Synaptic Transmission: Presynaptic Mechanisms

 

 

 

 

 

chemical transmission, quantum hypothesis, use-dependent synaptic plasticity

 

5 Nov F

 

22

Synaptic Transmission: Calcium and Transmitter Release

pp. 323-356

 

 

 

 

calcium hypothesis, molecular mechanisms of release, calcium domains

 

 

9 Nov T

 

23

Synaptic Transmission: Postsynaptic Mechanisms

 

 

 

synaptic conductances and reversal potentials, diversity of neurotransmitters, electrical synapses, dendritic spines

 

12 Nov F

 

24

Biophysical Methods Presentation #3

 

Discussion Leader: Paula Nieto & Lily Barret

Primary Literature

 

 

In Vivo Electrophysiology and Viral Infection Strategies

 

 

16 Nov T

25

Extracellular Field Recordings, Cellular Neurophysiology of

 

 

 

 

potentials in a volume conductor, classification of fields, current source-density analysis

 

19 Nov F

 

26

Biophysical Methods Presentation #4

Discussion Leader: Ryan Ochoa & Meizhu Qi

Primary Literature

 

 

Voltage Sensitive Dyes, Optical Sensors, and Optogenetics

 

23 Nov T

27

 

Biophysical Methods Presentation #5

Discussion Leaders: Ashley Loeven

Primary Literature

 

 

X-ray Crystallography/Cryo EM

 

26 Nov F

 

Thanksgiving Holidays

No Classes

 

 

 

30 Nov T

28

Learning and Memory

 

 

 

spine shape and synaptic plasticity, Hebb rule, cellular mechanisms of LTP & LTD, correlation matrix

 

3 Dec F

29

Ion Channel Presentation/Methods

 

Discussion Leader: Paula Nieto

Primary Literature

 

 

 

Nucleopore Complex

 

7 Dec T

 

Final Examination (Week of 10 14 Dec)

 

7:30 am to 10:30 am