3D Deconvolution Image Data from five different nuclei.
Two are from desyanptic1 (dsy1) pollen mother cells, and three are from desynaptic (dy).
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File Name Mutant Stage HBID Fig. Description
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dsy1_ZY_DFO.JPG [JPG]
dsy1_ZY_D [AVI][MOV] dsy1 Zygotene 5s3b 1A grey-scale sections, DAPI (DNA)
dsy1_ZY_F [AVI][MOV] " " " " grey-scale sections, FITC (telomeres)
dsy1_ZY_O [AVI][MOV] " " " " grey-scale sections, Rhod. (5S rDNA)
dsy1_ZY_DFO [AVI][MOV] " " " " color sections, DAPI/FITC/RHOD(5S rDNA) = Blue/Green/Red
dsy1_ZY_360 [AVI][MOV] " " " " color, spinning projections DAPI/FITC/RHOD(5S rDNA) = Blue/Green/Red
dsy1_DP_DO.JPG [JPG]
dsy1_DP_D [AVI][MOV] dsy1 Diplotene 5s3d - grey-scale sections, DAPI (DNA)
dsy1_DP_F [AVI][MOV] " " " - grey-scale sections, FITC (telomeres)
dsy1_DP_O [AVI][MOV] " " " - grey-scale sections, Rhod. (5S rDNA)
dsy1_DP_DFO [AVI][MOV] " " " - color sections, DAPI/FITC/RHOD = Blue/Green/Red
dsy1_DP_360 [AVI][MOV] " " " - color, spinning projections
DAPI/FITC/RHOD = Blue/Green/Red
dy_LE_DFO.JPG [JPG]
dy_LE_D [AVI][MOV] dy Leptotene 1t3e - grey-scale sections, DAPI (DNA)
dy_LE_F [AVI][MOV] " " " - grey-scale sections, FITC (telomeres)
dy_LE_O [AVI][MOV] " " " - grey-scale sections, Rhod. (5S rDNA)
dy_LE_DFO [AVI][MOV] " " " - color sections, DAPI/FITC/RHOD = Blue/Green/Red
dy_ZY_DFO.JPG [JPG]
dy_ZY_D [AVI][MOV] dy Zygotene 1t3d 2A grey-scale sections, DAPI (DNA)
dy_ZY_F [AVI][MOV] " " " " grey-scale sections, FITC (telomeres)
dy_ZY_O [AVI][MOV] " " " " grey-scale sections, Rhod. (5S rDNA)
dy_ZY_DFO [AVI][MOV] " " " " color sections, DAPI/FITC/RHOD Blue/Green/Red
dy_PA_DFO.JPG [JPG]
dy_PA_D [AVI][MOV] dy Pachytene 2t3e 2B grey-scale sections, DAPI (DNA)
dy_PA_F [AVI][MOV] " " " " grey-scale sections, FITC (telomeres)
dy_PA_O [AVI][MOV] " " " " grey-scale sections, Rhod. (5s rDNA)
dy_PA_DFO [AVI][MOV] " " " " color sections, DAPI/FITC/RHOD = Blue/Green/Red
dy_PA_360 [AVI][MOV] " " " " color, spinning projections,
DAPI/FITC red/green
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Table notes:
Single (JPG) images are greyscale projections of whole cell or nucleus subset images (DAPI, FITC, and Rhod.)
'grey-scale sections' show the entire 3D subset of data (optical sections) for a particular wavelength
(D for DAPI, F for FITC, and O for Rhodamine).
'color sections' show the entire 3D subset with psuedocolor overlay of all three wavelengths
'color, spinning projections' show through focus projections viewed from a series of angles about the Y axis,
these appear to spin when played through in sequence.
[AVI] are AVI movie format, [MOV] QuickTime movie format
Links to the paper J Exp Bot (2003) 54:39-46.
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