The Nerve Impulse Seen from Outside
Dexter M. Easton July 2000

Next topic   Previous Topic     Table of Contents

Topic 32. End-plate potential (EPP)

In frog skeletal muscle, each motor nerve fiber entering a skeletal muscle branches several times, and each branch terminates as a discrete structure, the end plate, on a muscle fiber. There may be hundreds of such motor units (neuron plus its muscle fibers) in a muscle. The currents associated with a nerve impulse dying in a nerve terminal pass through the membrane of the postsynaptic cell. At some synapses in the nervous systems of some animals, these currents can depolarize the postsynaptic cell to the threshold level required to yield a propagated impulse. In vertebrate skeletal muscle, the depolarization is accomplished mainly by a chemical synaptic transmitter, acetylcholine (Ach), which is ejected from the nerve terminal membrane. Ordinarily, the local depolarization (the end-plate potential, or EPP) is immediately overwhelmed by a propagated action potential (AP) in the post-synaptic cell.

Under ordinary circumstances, a single EPP in a frog muscle will be followed by an AP. When the Mg++ in solution is several times normal concentration, as in the "diluted sea water Ringer" used in these exercises, or when a poison like curare is present, a single impulse entering the nerve terminal may provoke only an EPP and no AP.

A single shock, S1, to the nerve, yields an EPP, which rises to a peak, then slowly decays. This is the response of many end plates activated not simultaneously but slightly dispersed in time. The record is rounded and prolonged compared to the response of a single end plate. Additional depolarization evoked by a second impulse, S2, delivered to the end plate at a suitable interval (here 20 msec), increases the local depolarization enough to trigger an action potential (AP). The EPP is monophasic because external currents flow only toward the end-plate zone from adjacent regions. The currents flow along the whole length of the muscle (Fig. 32B). The EPP decays with time, and by time S2' (here about 80 msec) little of it remains. Therefore, the late AP, depending mainly on the EPP triggered by S2 alone, is small.  


Next topic   Previous Topic    Table of Contents