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High-resolution 3D imaging technologies are becoming increasingly important for connecting genome regulation and nuclear architecture. Numerous specialized sub-nuclear compartments have been discovered and described in animals, but relatively little is known about the organization of interphase chromatin in plants. We propose to address a major challenge in biology, defining higher-order genome organization in a functional context. This aspect of the project aims to characterize changes in spatial organization of the maize genome by three-dimensional fluorescence microscopy in the context of transcriptional regulation and large-scale chromatin accessibility. |