Date:

 

Lecture#

 

Topic:

 

Text:

 

26 Aug T

1

 

Introduction

Basic Electricity

Hille Chapter 1;

 

Primary Literature

 

 

Course Overview; Selection of Assignments

definitions and calculations for electricity

 

27 Aug W

 

2

Electrical Circuits

Gerrish et al.

Chapters 1 & 6

 

 

definitions and calculations for circuits

 

29 Aug F

 

3

Measuring Biological and Optical Signals

Aidley

Chapters 1-3

 

 

 

 

amplifiers and voltage-clamp circuits, definitions of optical  probes and equipment

 

 

2 Sept T

 

4

Ion Movement in Excitable Cells

Hille Chapter 2

 

 

Nernst potential; Goldman-Hodgkin-Katz, physical laws that dictate ion movement

 

5 Sept F

 

5

 

Channel Presentation #1

Discussion Leaders:   Kirill Korshunov, Genevieve Bell, & Erminia                 Fardone

Primary Literature;

Hille Chapter 6

 

 

 

 

Transmitter or Ligand-gated Channels

 

 

12 Sept F

 

6

 

Channel Presentation #2

 Discussion Leaders:  Joe McKenna and Vehpi Yildirim

Primary Literature;

Hille Chapter 7

 

 

Second-messenger Gated Ion Channels

 

 

16 Sept T

7

 

Electrical Properties of the Excitable Membrane

Hille Chapter 3

 

 

 

 

biological circuits, membrane conductance, parallel conductance model, IV relationships

 

 

19 Sept F

 

8

 

Channel Presentation #3

 

Discussion Leaders:  Zhenbo Huang and Brandon Chelette

Primary Literature;

Hille Chapter 4

 

 

 

 

Non- Selective and Sodium Ion Channels

 

 

 

23 Sept T

 

9

 

Functional Properties of Dendrites

 

Primary Literature

 

 

 

 

electrotonic properties, nonisopotential cells (cylinders), rall model for neurons

 

26 Sept F

 

10

 

Channel Presentation #4

Discussion Leaders:  Diana Diaz and Dolly Al Koborssy

Primary Literature;

Hille Chapter 3, 9

 

 

 

 

Potassium Ion Channels

 

 

30 Sept T

 

11

 

Nonlinear Properties of Excitable Membranes

 

Hille Chapter 2

 

 

 

 

membrane rectification, Hodgkin & Huxley gate model, constant field model

 

 

3 Oct F

 

12

 

Channel Presentation #5

Discussion Leaders:  Austin Schwartz and Kassi Ferguson 

Primary Literature;

Hille Chapter 5

 

 

 

 

 

Chloride Ion Channels

 

 

7 Oct T

 

13

 

Squid Giant Axon

 

Hille Chapter 2

 

 

 

 

 

classical voltage-clamp, H & H model, propagating & nonpropagating AP, gating currents

 

10 Oct F

 

14

 

Channel Presentation #6

 Discussion Leaders:  Daniel Blackman and Thomas Arnold 

Primary Literature;

Hille Chapter 4

 

 

 

 

Calcium Ion Channels

 

 

14 Oct T

 

15

 

Functional Diversity of Voltage-gated Conductances

Hille Chapter 5, 13

 

 

 

 

properties and functions of membrane currents

 

 

15 Oct W

16

 

Biophysical Methods Presentation #1

 

Primary Literature

 

 

 

Patch-clamp Electrophysiology

 

Discussion Leader:  Debi Fadool

Sakmann/Neher Chapter 1

21 Oct T

 

17

 

Molecular Structure and Unitary Currents of Ion Channels

Hille Chapter 13,

Sakmann/Neher Chapter 3,18, and 19

 

 

 

 

molecular structure, patch-clamp records of single-channel currents

 

 

24 Oct F

 

18

 

Biophysical Methods Presentation #2

Discussion Leader:  Daniel Blackman and Brandon Chelette

Primary Literature

 

 

 

 

 

 

Optogenetics

 

28 Oct T

 

19

 

Stochastic Analysis of Single-Channel Function

Sakmann/Neher Chapter

18 and 19

 

 

 

 

statistical analysis of single channel records, probability density functions, Markov processes, kinetic schemes, channel agonists & antagonists

 

 

 

30 Oct F

 

20

 

Biophysical Methods Presentation #3

 

Discussion Leader:  Vehpi Yildirim, Diana Flores, and Dolly Al Koborssy

Primary Literature

 

 

 

Dynamic Clamp Techniques

 

4 Nov T

 

21

 

Synaptic Transmission:  Presynaptic Mechanisms

 

 

 

 

chemical transmission, quantum hypothesis, use-dependent synaptic plasticities

 

 

7 Nov F

 

22

 

Biophysical Methods Presentation #4

Discussion Leaders: Thomas Arnold and Erminia Fardone

Primary Literature

 

 

 

 

MRI

 

11 Nov T

 

Veteran’s Day

No Classes

14 Nov F

 

23

 

Biophysical Methods Presentation #6

Discussion Leaders:  Kassi Ferguson and Austin Schwartz

Primary Literature

 

 

 

 

Crystallography

 

15-19 Nov

 

SFN Lectures and Presentations

 

21 Nov F

24

Biophysical Methods Presentation #5

 

Discussion Leader:  Kirill Korshunov and Joseph McKenna

Primary Literature

 

 

 

 

In Vivo Recording Approaches and Viral Infection Strategies

 

25 Nov T

 

25

 

Synaptic Transmission:  Calcium and Transmitter Release

pp. 323-356

 

 

 

 

calcium hypothesis, molecular mechanisms of release, calcium domains

 

 

27 Nov F

 

Thanksgiving Holidays

No Classes

2 Dec T

26

 

Synaptic Transmission:  Postsynaptic Mechanisms

 

 

 

synaptic conductances and reversal potentials, diversity of neurotransmitters, electrical synapses, dendritic spines

 

 

5 Dec F

 

27

 

Extracellular Field Recordings, Cellular Neurophysiology of

Learning and Memory

 

 

 

 

 

 

potentials in a volume conductor, classification of fields, current source-density analysis

spine shape and synaptic plasticity, Hebb rule, cellular mechanisms of LTP & LTD, correlation matrix

 

 

9 Dec T

 

 

 

Final Examination (Week of 8 – 12 Dec)

 

10 am to 1 pm