FSU-NimbleGen Microarray Facility


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Microarray Serivce

I. Overview

The way our facility works is simple. You order the arrays of choice from NimbleGen through a normal purchase order. Your arrays are delivered to FSU. We notify you that we have them. You get the samples to us and we give you back a DVD with your data on it and a software viewer to browse your data.

Full service: includes sample labeling, sample hybridization, post hybridization washing, scanning and data extraction.

Partial service: coordinated with each individual researcher's needs to include training in any of the processing steps available in the full service option.

II. How do I order my arrays?

If you want to use an existing platform:

  • Go to http://www.nimblegen.com/.
  • Click on the application you wish to use.
  • Each application has a slightly different path to follow, but basically you will either go next to a kingdom and species (gene expression), or you will go to an array type and then see if your species is available in that array type (CGH, ChIP-chip, DNA methylation, and Gene Expression).
  • Request a quote from Michael Schilling mschilling@nimblegen.com that will indicate "FSU NimbleGen Certified Site" – you must identify yourself as an FSU Core user.
  • Set up a purchase order.
  • Order your arrays.
  • They should be on campus within 2-3 weeks.
  • Notify FSU Core that you have placed the order.

III. How do I process my sample?

You should arrange to have your samples ready close to the time when the arrays are expected to arrive. Make certain that you provide each sample with a unique ID. You may then submit your samples to the FSU Core. When the arrays arrive on campus, we will know which sample it belongs to and will hybridize them accordingly. We will then contact you when the DVD with your data is ready.

A WORKORDER sheet is required for every sample set. Make sure you include the following information:

  1. Your name, phone number, Department address, and e-mail address.
  2. A budget number must be supplied at the time the workorder is submitted. Non-FSU users need to submit a copy of their PO with their samples. No exceptions will be allowed.
  3. Template information:
    1. Name of the template
    2. Sample concentration, solvent, and quantity. Please write the concentration in terms of ug/ml, ug/ul, or ng/ul, NOT in terms of the total volume, such as 5.2 ug/320 ml.

Note: we will check your sample for quality control and if there is a problem with your sample, we will contact you. It will be your responsibility to either go ahead with a bad sample, or replace your sample with one that can pass quality control checks. We will do the best we can to advise you of what is wrong with your sample.

IV. How much sample do I need?

  • Transcription analysis: 2.5µg of cDNA at >100ng/µl, A260/280 >1.8 and A260/230 >1.8
  • ChIP-chip or CGH: ~3µg of DNA at >250ng/µl and A260/280 >1.8 and A260/230 >1.8

V. How much does it cost?

We are in the process of setting up a pricing schedule. Please contact us to obtain current prices. You must purchase your arrays from NimbleGen, at the price they quote for the application you desire.

VI. Can I process my own sample?

Yes, we will give you a discount per hybridization if you learn to process your own samples in house.