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• Construction of Genomic DNA Library: Purified genomic DNA of high molecular weight should be supplied by the investigator. The library will be constructed in a lambda vector of your choice and will be amplified.

Cost $700.00
Note: If the kit used for constructing the library is shared between two investigators the cost will be adjusted accordingly.

• Construction of cDNA Library: Purified total RNA should be provided by the investigator in sufficient quantity. We will isolate the mRNA and construct the cDNA library in lambda vector of your choice and amplify the library.

Cost $850.00
Note: If the kit used for constructing the library is shared between two investigators the cost will be adjusted accordingly.

• Screening of libraries: Libraries can be screened using oligonucleotide or double stranded DNA probes or antibodies.

Cost $80.00 per 500,000 plaques

• Subcloning:
  a. DNA fragments will be subcloned into the vector of your choice and recombinant clones will be verified by restriction analysis.
  b. PCR amplified DNA fragments will be subcloned in the TA cloning vector, pDK101. Recombinant clones will be verified by restriction fragment analysis.

Cost $30.00

• Plasmid vectors suitable for subcloning: Following is a list of currently abailable vectors.

pGEM 3Zf(-) and f(+)
pGEM 7Zf(-)
pBluescript SK+
pBC
Cost $1.00/ug

•  pDK101 vector for TA cloning: This vector is CsCl2 purified, digested with Xcm I to generate "T" overhangs and HPLC purified.

Cost $9.00/ug (sufficient for 10 subcloning reactions)

• Molecular weight markers: pGem 3Zf(-); 14 restriction fragments ranging in size from 22 bp to 2977 bp.

Cost $12.50/ tube 12.5 ug

• Competent E. coli for transformation: JM 109 and DH5alpha are available at all times. All other strains can be made available upon request.

Cost $1.50/ml

• Primers: The following primers are available for a nominal cost. and aother commonly used primers can be made available upon request.

T3 Primer
T7 Primer
M13 Forward Primer
M13 Reverse Primer
Oligo dT

• RT PCR: We have successfully reverse transcribed and PCR amplified specific gene sequences from total RNA and mRNA. If you are interested in trying this technique please contact us for further details.
  
• Enzymes, Reagents, and Molecular Products: We operate a Life Technologies (Gibco/BRL) Supply Center that is stocked with enzymes, reagents, and other molecular products.
  
• Workshops: The MCF conducts workshops each semester on various molecular biology related techniques. All interested faculty, staff, and graduate students are welcome to participate in these workshops. A memo will be circulated prior to each workshop with all pertinent information.