Dr. Fanxiu Zhu —FSU Biological Science Faculty Member -->

Dr. Fanxiu Zhu

Office: 207 Biology Unit I
Office: (850) 644-6273
Lab: Biology Unit I
Lab: (850) 645-7695
Fax: (850) 644-0481
Mail code: 4370
E-mail: fzhu@bio.fsu.edu

Associate Professor
Ph.D., Wuhan University, 1995
Graduate Faculty Status

POSITIONS AVAILABLE for postdoctoral investigators, graduate students, and undergraduate students.

Research and Professional Interests:

My lab studies Kaposiís sarcomaĖassociated herpesvirus (KSHV), a human DNA tumor virus associated with several human malignancies, including Kaposiís sarcoma, primary effusion lymphoma, and multicentric Castlemanís disease. I am interested in viral proteins that are localized in the tegument layer, a space between capsid and envelope in the virus particle. The tegument comprises at least a dozen proteins that are delivered into cells upon infection, so they have the opportunity to exert their functions at the very beginning in the infection process. The unique temporal and spatial expressions of tegument proteins put them in the forefront of coping with the host cellular environment. We discovered that KSHV tegument protein ORF45 interacts with interferon regulatory factor 7 (IRF-7) and antagonizes host antiviral response. Recently, we found that ORF45 also interacts with p90 ribosomal S6 kinases (RSKs) and modulates the ERK/RSK MAPK signaling pathway, which plays critical roles in KSHV lytic replication. We are now exploring the mechanisms by which ORF45 evades innate immune response and modulates ERK/RSK signaling. We are also interested in the structural analysis of herpesvirus tegument by cryoEM. The structural organization and morphogenesis of herpesviral tegument are largely unknown. We use various approaches to investigate why and how viral proteins are selectively assembled into the tegument.

Selected Publications:
  1. Liang, Q., B. Fu, F. Wu, X. Li, Y. Yuan, and F. Zhu. 2012. ORF45 of Kaposi's sarcoma–associated herpesvirus inhibits phosphorylation of interferon regulatory factory 7 by IKKε and TBK1 as an alternative substrate. Journal of Virology 86:10162-10172. PDF

  2. Kuang, E., B. Fu, Q. Liang, J. Myoung, and F. Zhu. 2011. Phosphorylation of eukaryotic translation initiation factor 4B (eIF4B) by open reading frame 45/p90 ribosomal S6 kinase (ORF45/RSK) signaling axis facilitates protein translation during Kaposi sarcoma-associated herpesvirus (KSHV) lytic replication. J. Biol. Chem. 286:41171-41182. PDF

  3. Liang Q, H. Deng, X. Li, X. Wu, Q. Tang, T. H. Chang, H. Peng, F. J. 3rd. Rauscher, K. Ozato, F. Zhu. 2011. Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 ligase and negative regulator of IFN regulatory factor 7. J. Immunol. 187:4754-4763. PDF Featured in this issue

  4. Liang, Q., H. Deng, C.-W. Sun, T. M. Townes, and F. Zhu. 2011. Negative regulation of IRF7 activation by ATF4 suggests a cross regulation between the interferon responses and the cellular integrated stress responses. J. Immunol. 186:1001-1010. PDF

  5. Sathish N., F. X. Zhu, E. E. Golub, Q. Liang, Y. Yuan. 2011. Mechanisms of auto-inhibition of IRF-7 and a probable model for inactivation of IRF-7 by Kaposi's sarcoma-associated herpesvirus protein ORF45. J. Biol. Chem. 286:746-756. PDF

  6. Zhu, F. X., N. Sathish, Y. Yuan. 2010. Antagonism of host antiviral responses by Kaposi's sarcoma-associated herpesvirus tegument protein ORF45. PLoS One 5:e10573. PDF

  7. Kuang, E., F. Wu, and F. Zhu. 2009. Mechanism of sustained activation of ribosomal S6 kinase (RSK) and ERK by Kaposi sarcoma-associated herpesvirus ORF45: multiprotein complexes retain active phosphorylated ERK and RSK and protect them from dephosphorylation. J. Biol. Chem.284:13958-13968. PDF

  8. Satish, N., F. X. Zhu. and Y. Yuan. 2009. Kaposiís sarcoma-associated Herpesvirus ORF45 interacts with kinesin-2 transporting viral capsid-tegument complexes along microtubules. PLoS Pathog.5:e1000332. PDF

  9. Li, X., and F. Zhu. 2009. Identification of the nuclear export and adjacent nuclear localization signals for ORF45 of Kaposi's sarcoma-associated herpesvirus. J. Virol. 83:2531-2539. PDF

  10. Kenworthy, R., D. Lambert, F. Yang, N. Wang, Z. Chen, H. Zhu, F. Zhu, C. Liu, K. Li, and H. Tang. 2009. Short hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation. Nucleic Acids Res. 37:6587-6599. PDF

  11. Kuang, E., Q. Tang, G. G. Maul, F. Zhu. 2008. Activation of p90 ribosomal S6 kinase by ORF45 of Kaposi's sarcoma-associated herpesvirus and its role in viral lytic replication. J. Virol. 82:1838-1850. PDF

  12. Zhu, F. X., X. Li, F. Zhou, S.-J. Gao, and Y. Yuan. 2006. Functional characterization of Kaposi's sarcoma-associated Herpesvirus ORF45 by BAC-based mutagenesis. J. Virol. 80:12187-12196. PDF

  13. Zhu, F. X., J. M. Chong, L. Wu, and Y. Yuan. 2005. Virion proteins of Kaposi's sarcoma-associated herpesvirus. J. Virol. 79:800-811. PDF

  14. Wang, Y., H. Li, M. Y. Chan, F. X. Zhu, D. M. Lukac, and Y. Yuan. 2004. Kaposi's sarcoma-associated herpesvirus ori-Lyt-dependent DNA replication: cis-acting requirements for replication and ori-Lyt-associated RNA transcription. J. Virol. 78:8615-8629. PDF

  15. Zhu, F. X., and Y. Yuan. 2003. The ORF45 protein of Kaposi's sarcoma-associated herpesvirus is associated with purified virions. J. Virol. 77:4221-4230. PDF

  16. Lin, C. L., H. Li, Y. Wang, F. X. Zhu, S. Kudchodkar, and Y. Yuan. 2003. Kaposi's sarcoma-associated herpesvirus lytic origin (ori-Lyt)-dependent DNA replication: identification of the ori-Lyt and association of K8 bZip protein with the origin. J. Virol. 77:5578-5588. PDF

  17. Biswas, S. B., S. M. Khopde, Fx. F. Zhu, E. E. Biswas. 2003. Subunit interactions in the assembly of Saccharomyces cerevisiae DNA polymerase α. Nucleic Acids Res. 31:2056-2065. PDF

  18. Zhu, F. X., S. M. King, E. J. Smith, D. E. Levy, and Y. Yuan. 2002. A Kaposiís Sarcoma-associated herpesviral protein inhibits virus mediated induction of type I interferon by blocking IRF-7 phosphorylation and nuclear accumulation. Proc. Natl. Acad. Sci. USA 99:5573-5578. PDF

  19. Saveliev, A., F. X. Zhu, and Y. Yuan. 2002. Transcription mapping and expression patterns of genes in the major immediate-early region of Kaposi's sarcoma-associated herpesvirus. Virology 299:301-314. PDF

  20. Zhu, F. X., T. Cusano, and Y. Yuan. 1999. Identification of immediately early genes of Kaposiís sarcoma herpesvirus. J. Virol. 73:5556-5567. PDF

  21. Sun, R., S.-F. Lin, L. Gradoville, Y. Yuan, F. Zhu, and G. Miller. 1998. A viral gene that activates lytic cycle expression of Kaposi's sarcoma-associated herpesvirus. Proc. Natl. Acad. Sci. USA 95:10866-10871. PDF

  22. Zhu, F. X., E. E. Biswas, S. B. Biswas. 1997. Purification and characterization of the DNA polymerase α associated exonuclease: the RTH1 gene product. Biochemistry 36:5947-5954. PDF

  23. Biswas, E. E., F. X. Zhu, S. B. Biswas. 1997. Stimulation of RTH1 nuclease of the yeast Saccharomyces cerevisiae by replication protein A. Biochemistry 36:5955-5962. PDF

  24. Zhu, F. X., Y. P. Qi, Y. X. Huang, Q. Bing. 1996. A baculovirus vector derived from immediately early gene promoter of Autographa californica nuclear polyhedrosis virus. J. Virol. Methods 62:71-79. PDF

Postdoctoral Associates:

Li, Wenwei
Wu, Jianjun

Graduate Students:

Avey, Denis
Brewers, Brittany
Frausto, Stephen
Gillen, Joseph
Ma, Siming

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