Study Questions 2

October 8- October 24

What are the three components of receptor mediated endocytosis? Briefly describe the component as used in the uptake of LDL.

What are the functional domains of the LDL receptor?

How does the cell ensure that only certain ligands are internalized by receptor mediated endocytosis? e.g. what proteins are present to “specify” what comes in?

Outline the process of LDL internalization.

What protein links the LDL receptor to clathrin?

Briefly outline the experiment of Brown and Goldstein.

Give two general examples of switches used in cell signaling.

Briefly outline the events of signaling in response to adrenaline.

What is the difference between a GEF and a GAP?

Outline or draw the events of EGF signaling, including the names of the major components.

Why is the process of amplification important for cell signaling, particularly in the adrenaline response?

What is the cellular function and location of the E.R.?

What is the function of the Golgi and how does it structure relate to this function?

What is the function of the N terminal signal sequence?

What is the function and location of signal peptidase?

What is the function and location of the signal recognition particle?

What are the three types of topogenic sequences and what do they do?

How and where are N-linked glycoproteins formed?

What is the function and location of dolichol?

What are the five destinations of proteins from the Golgi?

How are E.R. proteins retained in the E.R.?

Breifly outline the experiment of Munroe and Pelham.

Briefly describe two ways that it was determined that N terminal sequences in mitochondrial proteins lead to their import into the mitochondria?

What is the function and location of both TOM and TIM family proteins?

What are the three properties characteristic of protein import into the mitochondria? How were these properties proven experimentally?

What is the function of chaperones? What cellular small molecule do they require for their function?

What type of sequence is required for a protein to be imported to the matrix?

How does the process of electron transport in the inner membrane relate to the import of mitochondrial proteins?